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KMID : 0613820230330030234
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Journal of Life Science
2023 Volume.33 No. 3 p.234 ~ p.241
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Antioxidant Activity of Cannabidiol (CBD) and Effect on Its Proliferation in Human Dermal Papilla Cells
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Kim Soo-Hyun
Sim Kyu-Sang
Cheon,Jung-Yoon
Jang Jae-Woong
Jeong Su-Jin
Seo Ye-Hei
Ahn Hye-Myoung
Song Bong-Geun
Kwon Gi-Seok
Lee Jung-Bok
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Abstract
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At present, many countries around the world are legalizing cannabis and its products, and research on various treatments using cannabis is being actively conducted. However, the cannabis plant contains other compounds whose biological effects have not yet been established. We investigated the effect of cannabidiol (CBD) on hair growth in human dermal papilla cells (HDPCs). 2,2′-Azino-bis (3-ethylbenzothiazolin-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays were performed to determine the antioxidant activity of CBD. The HDPCs viability of CBD was examined via water-soluble tetrazolium salt (WST-1) assay. The expression of hair-loss-related markers in HDPCs by CBD treatment was analyzed by real-time PCR and western blotting. The DPPH, ABTS radical scavenging activity assay showed that CBD had superior antioxidant activities. In HDPCs, CBD increased cellular proliferation at concentrations without cytotoxicity. It also increased the expressions of fibroblast growth factor 1 (FGF1), fibroblast growth factor 7 (FGF7), vascular endothelial growth factor (VEGF), and insulin-like growth factor (IGF). These results correlated with a decrease in the expression of inhibition-related factors, such as androgen receptor (AR) and transforming growth factor beta 1 (TGF-B1). Moreover, CBD resulted in a significant increase in the phosphorylation of AKT and extracellular signal-regulated kinase (ERK). Therefore, it is suggested that CBD may be a potential remedy for the treatment of alopecia.
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KEYWORD
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Antioxidant, cannabidiol, hemp, human dermal papilla cells, proliferation
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